A431	human epidermoid carcinoma cell line
A498	human renal cell carcinoma cell line
A549	human lung carcinoma cell line
aa	amino acid
BSA	bovine serum albumin
CA	carbonic anhydrase
CA IX	carbonic anhydrase IX
CA IX+PG	recombinant full-length extracellular domain of CA IX
CA IX-PG	recombinant catalytic domain without PG region
CA XII	carbonic anhydrase XII
CaSki	human cervical squamous cell carcinoma cell line
DMEM	Dulbecco’s modified Eagle’s growth medium
ELISA	enzyme-linked immunosorbent assay
FBS	fetal bovine serum
HAT	hypoxanthine/aminopterin/thymidine
HEK-293	human embryonic kidney 293 cell line
HeLa	human cervical adenocarcinoma cell line
HRP	horseradish peroxidase
HT29	human colorectal carcinoma cell line
IFA	immunofluorescence assay
IgG	immunoglobulin G
IHC	immunohistochemistry
IP	immunoprecipitation
Jurkat	human T cell leukemia cell line
Kd	apparent dissociation constant
MAbs	monoclonal antibodies
MCF-7	human breast adenocarcinoma cell line
MDA-MB-231	human triple-negative breast cancer cell line
OD	optical density
PBS	phosphate-buffered saline
PBS-T	0.1% Tween 20 in PBS buffer
PBS-TW	PBS supplemented with 0.05% of Tween 20
PG	proteoglycan region of CA IX
PPV	porcine parvovirus
PVDF	polyvinylidene difluoride
RT	room temperature
SDS	sodium dodecyl sulfate
SDS-PAGE	sodium dodecyl sulfate-polyacrylamide gel electrophoresis
TBS-T	Tris buffered saline with 0.5% Tween 20
TMB	3,3′,5,5′-Tetramethylbenzidine
U-87	human glioblastoma cell line
WB	Western blot