Table 2.
List of antibodies used in this study. FITC: fluorescein-isocyanate; HRP: horseradish peroxidase; ZO-1: zonula occludens-1.
| Antibody | Antigen | Immunogen | Host | Source, Cat. # |
|---|---|---|---|---|
| Anti-MUC5AC Antibody (45M1) | Peptide core of gastric mucin M1 (Mucin 5AC) | M1 mucin | mouse | Novus biologicals, Centennial, CO, USA, Cat. #NBP2-15196 |
| Anti-ZO-1 (ZMD. 437) | ZO-1 | synthetic peptide derived from the N-terminal region of human, dog, mouse, and rat ZO-1 | rabbit | Thermo Fisher Scientific, Dreieich, Germany, Cat. #40-2300 |
| Anti-acetylated tubulin (6-11B-1) | Acetylated tubulin | acetylated tubulin from the outer arm of Strongylocentrotus purpuratus | mouse | Sigma Aldrich, Taufkirchen, Germany, Cat. #T7451 |
| Anti-β Actin (AC-15) | β Actin | not specified | mouse | Sigma Aldrich, Taufkirchen, Germany, Cat. #A5441 |
| Anti-murine IgG-Alexa Fluor® 488 | whole molecule mouse IgG | not specified | Goat | Jackson Immuno Research Europe Ltd., Cambridgeshire, UK, Cat. #115-545-003 |
| Anti-rabbit IgG-Rhodamine Red™-X | whole molecule rabbit IgG | not specified | donkey | Jackson Immuno Research Europe Ltd., Cambridgeshire, UK, Cat. #711-295-152 |
| Anti-murine IgG-HRP | whole molecule mouse IgG | not specified | goat | Sigma Aldrich, Taufkirchen, Germany, Cat. #AP5278 |
| Anti-rabbit IgG-HRP | Whole molecule rabbit IgG | not specified | Goat | Jackson Immuno Research Europe Ltd., Cambridgeshire, UK, Cat. #111-035-003 |