Protein composition and IgE reactivity of Artemisia spp. pollen extracts. (a) Protein extracts (8 µg per lane) were separated by denaturing gel electrophoresis; (b) IgE reactivity to Artemisia extracts was evaluated in an ELISA using sera of mugwort pollen-allergic patients (n = 11). Boxes indicate the interquartile ranges, the median is shown as a solid line, and whiskers represent the 5th and 95th percentile. One-way ANOVA using Tukey’s post hoc test was used for statistical analysis. ABS, A. absinthium; ANN, A. annua; CAL, A. californica; FRI, A. frigida; LUD, A. ludoviciana; TRI, A. tridentata; VUL, A. vulgaris.