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Mediator release assay. Rat basophil leukemia (RBL) cells transfected with an anti-human IgE high-affinity receptor (RBL-2H3) were sensitized with four mugwort-allergic patients’ sera (P1–P4). Cells were stimulated with increasing concentrations of purified allergens, and the release of β-hexosaminidase was measured and is expressed as a percentage of total release with Triton X-100.
