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The Art v 125–36-specific TCR cross-reacted with purified DPLP proteins presented on APCs. HLA-DR1+ artificial APCs were pre-incubated for 2 h with purified DPLPs, and Art v 125–34 peptides were cocultured with TCRtg Jurkat cells for 6 h before luciferase activity was determined. Data show mean values of triplicate cultures representative of two independently performed experiments.
