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. 2019 Aug 15;11(8):756. doi: 10.3390/v11080756

Figure 1.

Figure 1

Figure 1

HSV-1 infection attenuated cytosolic DNA sensor reporter expression. (A) The scheme of dual-luciferase reporter plasmid construction. pmirGLO vector was digested with SacI and XbaI, and then inserted into full-length 3’-UTR sequences of different mRNAs with a correct linker connected with T4 DNA ligase. Hsa-miR-16 complement (pmirGLO-16), as a positive internal control, was constructed by cloning complete complementary sequences into pmirGLO vector. (B) Different CPEs of HKE-293T cells infected with HSV-1 at different viral titers and times. HEK-293T cells were infected with HSV-1 at an MOI of 0.1, 1, 10, and 50 or Mock and snapped at the indicated time points. Scale bar, 100 µm. (C,D) HEK-293T (C) or THP-1 (D) cells were transfected with a reporter plasmid, and then infected with HSV-1 or mock. The cells were transfected with 500 ng indicated reporter plasmid. After 24 h of transfection, the cells were infected with HSV-1 at an MOI of 1 or mock for another 24 h, collected, lysed, and assayed for dual-luciferase activity. The values present the percentage of reporter activity with infection compared with that with mock infection, standardized to 100% in the empty vector (EV) group. Data are the means ± SD (n = 3) from one representative experiment. Similar results were obtained from three independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001.