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. 2019 Jul 31;11(8):1764. doi: 10.3390/nu11081764

Figure 1.

Figure 1

Reactive oxygen species (ROS) generation by lymphoblast cell lines in response to acute exposure (1 h) to oxidizing agents measured by 2′7′-dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay. (a) H2O2–stimulated ROS. One h of treatment with H2O2 induced oxidative stress, showing significant greater ROS generation in Alzheimer’s disease (AD) than in healthy control (HC) lymphoblasts. Two-way ANOVA indicated an effect of both treatment (Tr) (F(3,16) = 19.52; p < 0.0001) and disease (Ds) (F(1,16) = 17.21; p < 0.001); (b) FeSO4-stimulated ROS. FeSO4 induced oxidative stress. Two-way ANOVA indicated effects of both Tr (F(3,16) = 122.2 and p < 0.0001) and Ds (F(1,16) = 13.23; p < 0.01); and an interaction Tr × Ds (F(3,16) = 5.121; p < 0.05). Values are mean ± SEM of three independent experiments with n = 3–5/experiments on two different cell lines per group. P-values of Tukey’s post hoc tests for each experimental condition relative to the control treatment within each group (AD or HC) are presented as: * p < 0.05, ** p < 0.01, and *** p < 0.001.