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. 2019 Jul 31;11(8):1764. doi: 10.3390/nu11081764

Figure 3.

Figure 3

Relative expression of genes involved in oxidative stress in AD and HC lymphoblast cell lines treated with selenite (Se (IV)), selenate (Se (VI)), and resveratrol (RV). Gene expression analysis by real-time PCR from lymphoblast mRNA using TaqMan Fluorescein amidite (FAM)-labeled specific probes and normalized with the mean of both housekeeping genes: phosphoglycerate kinase 1 (PGK1) and beta-2-microglobulin (B2M). (a) Catalase (CAT); (b) copper chaperone for SOD1 (CCS); (c) alpha galactosidase (GLA); (d) glutathione peroxidase 1 (GPX1); (e) glutathione peroxidase 4 (GPX4); (f) glutathione reductase (GSR); (g) glutathione S-transferase zeta 1 (GSTZ1); (h) nuclear factor (erythroid-derived 2)-like 2 (NEF2L2); (i) peroxiredoxin 5 (PRDX5); (j) superoxide dismutase 2 (SOD2); (k) thioredoxin interacting protein (TXNIP). P-values for two-way ANOVA analysis are indicated at the top or right area of the graph. Tr: treatment effect; Ds: disease effect. P-values of Tukey’s post hoc tests for each group (relative to control treatment) are indicated in the graphs as: + p < 0.1, * p < 0.05, ** p < 0.01, **** p < 0.0001. Values are mean ± SEM of two to four independent experiments with n = 2/experiment on two different cell lines per group. HC and AD stand for healthy control and Alzheimer’s disease, respectively.