Figure 1.

HFn recognition of BC cells. BT474, SKBR3, and MCF-7 cells were incubated for 1 h at 37 °C in complete cell culture medium with different amounts of FITC-labelled HFn nanoparticles (10 and 100 μg/mL). Then, cells were collected, washed, and processed for flow cytometry, using untreated cells to set the positive and the singlet gates. Reported values are the means ± s.e. (n = 3).