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Transactivation activity assay of CmNAC60 in yeast cells. (A) Diagram showing that full-length protein (CmNAC60-FL), N-terminal fragment (CmNAC60-N), and C-terminal fragment (CmNAC60-C) of CmNAC60 were fused with a GAL4 DNA binding domain. The pBridge vector was used as a negative control. (B) The transformed yeasts were dripped on the SD/-Trp, SD/-His, and SD/-His supplemented with X-α-Gal medium, respectively.
