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. 2019 Aug 19;9(8):380. doi: 10.3390/biom9080380

Figure 2.

Figure 2

Figure 2

Protective effect of hispidin against H2O2-induced oxidative stress on ARPE-19 cells. (A) ARPE-19 cells were pre-treated with various concentrations (2.5–20 μM) of hispidin for 24 h and then treated with 300 μM H2O2 for 6 h. The H2O2-induced reactive oxygen species (ROS) generation was measured by dichlorofluorescin diacetate (DCFDA) assay. The green fluorescence represents the cells stained for ROS (magnification 200x); (B) Fluorescence was then measured with a multi-well fluorescence reader at 535 nm (emission). ** p < 0.01 versus vehicle control.