Table 1.

miR159 targets in Arabidopsis as determined by different approaches. The top 20 miR159a targets in Arabidopsis as identified by the bioinformatic program psRNATarget with standard search parameters [40]; the number of mismatches is indicated by the score. Confirming this prediction, 5′-RACE analysis can detect miR159-guided cleavage products for at least nine of these genes. In contrast, the more quantitative degradome analysis only identifies three of these genes, with only MYB33 and MYB65 being frequently detected in multiple degradome analyses [25,42]. Overexpression of miR159 could detect down-regulation of multiple targets [13,52]. However, genetic analysis using a loss-of-function mir159ab mutant identify MYB33 and MYB65 as the major important targets [14,41].

	At Number	Score	Name	5’-RACE	Degradome	miR159 OE	miR159ab
1	AT4G37770	1.5	ACS8	[53]		[13]
2	AT2G32460	2	MYB101	[15,17,53]		[13]
3	AT3G60460	2	DUO1	[15,17,53]
4	AT2G26950	2	MYB104
5	AT4G26930	2	MYB97
6	AT5G06100	2.5	MYB33	[15,23]	[25,42]	[52]	[14,41]
7	AT3G11440	2.5	MYB65	[23]	[25,42]		[14,41]
8	AT2G34010	2.5	MRG1	[53]	[42]
9	AT2G21600	2.5	RER1B
10	AT5G55020	2.5	MYB120	[15]		[13]
11	AT4G27330	2.5	SPL
12	AT5G27395	2.5	Tim44-related
13	AT3G61740	3	SDG14, ATX3
14	AT1G29010	3	MRG-LIKE
15	AT4G31240	3	NRX2
16	AT2G26960	3	MYB81	[15]
17	AT2G22810	3	ACS4
18	AT3G08850	3	RAPTOR1B
19	AT5G55930	3.5	OPT1	[13]		[13]	[41]
20	AT2G44450	3.5	beta gluc 15