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In vitro assays of oat HHT gene (AsHHT1) using the protein expressed in E. coli. HPLC analysis of the products in the presence of 5-hydroxy-anthranilic acid with p-coumaroyl-CoA (A) and with caffeoyl-CoA (C). The negative controls with boiled AsHHT enzymes were shown in (B,D) with corresponding substrates.
