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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Nature. 2019 Apr 8;569(7756):361–367. doi: 10.1038/s41586-019-1127-1

Figure 5: Spatial patterning and organ identities within the E8.75 gut tube of the mouse embryo.

Figure 5:

a, Force directed graph of E8.75 cells colored by Phenograph clusters, annotated with putative endodermal organ associated with each cluster. b, Density of cells, per Phenograph cluster along AP pseudo-space. c, Percentage VE cells per cluster, ordered by average distance from anterior tip of AP pseudo-space. d, Heatmap of Hox gene expression along AP pseudo-space (left). Validation of Hox gene expression by ISH on E8.75 gut tubes (n >3 for each gene) (right). e, Heatmap of TF expression most predictive of AP pseudo-space in a regression model. Columns represent cells ordered by pseudo-space, each row representing expression of a particular TF. TFs ordered by expression along AP pseudo-space. Validation of predictive AP expression by ISH on E8.75 gut tubes (n >3 for each gene) (right). Scale bars: 200μm, except for Nkx2–1, Irx3: 100μm. A, anterior; cm, cardiac mesoderm; fg, foregut; hg, hindgut; L, left; mg, midgut; R, right; P, posterior. Scale bars: 200μm, except for Hoxc10: 100μm.

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