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. 2019 Sep 4;18:151. doi: 10.1186/s12934-019-1202-1

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Maximal extracellular acetate concentrations. a Maximal acetate concentrations detected for E. coli BL21 wt and knockout strains growing in MM9 or TB7 medium supplemented with glucose or glycerol as the carbon source. b Acetate extracellular concentration percentage relative to E. coli BL21 wt growing on glucose-TB7. c Extracellular acetate concentrations/q_s ratio expressed as a percentage relative to E. coli BL21 wt growing on glucose-TB7. Statistical tests, including two-way ANOVA (a and c) and one-way ANOVA (b), were carried out in order to evaluate differential significance between knockout mutants and the wt strain in each condition (a and c), and the differential significance between glucose-TB7 and the other media in the wt strain (b) (p-value < 0.0001 (****), < 0.001 (***), < 0.01 (**), < 0.05(*))