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. 2019 Sep 3;19:875. doi: 10.1186/s12885-019-6071-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — TEX9 is associated with eIF3b expression. a Comparsion of proteomics data for eIF3b-depleted (sh1 and sh2, technical replicate), eIF3b-overexpressed (ov1 and ov2, technical replicate) and normal control of EC109 cells (NC1 and NC2, technical replicate). b Relative quantification 1.5 fold-changes and p-value < 0.05 were selected as being differentially expressed. Significantly up- and down-regulated proteins in eIF3b-overexpressed cells were highlighted in red and blue, respectively. c Western blot assay was performed to detect the TEX9 and eIF3b expression in eIF3b-depleted, eIF3b-overexpressed and normal control of EC109 cells. Tubulin was used as an internal reference. d RNA-IP was performed to verify that eIF3b can bind to TEX9 mRNA in EC109 cells. The protein level was detected by Western blot assay and the mRNA level was detected by RT-PCR. “NC”, normal control. “sh”, eIF3b-depleted. “ov”, eIF3b-overexpressed. (ns: no significance, *p < 0.05, **p < 0.01, ***p < 0.001)