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. 2019 Sep 3;7:144. doi: 10.1186/s40478-019-0794-3

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Comparison of human brain samples with different PMIs prepared with standard embedding and hybrid freezing. Overview micrographs (3 × 3 montages) at low magnification (1500x) (a-d) samples with increasing PMI (case 1–4) prepared with standard embedding method; e-h Samples of the same area from the same cases processed with cryo-substitution cocktail I, hybrid freezing. Scale bars = 1 μm