Fig. 4.

Neuronal loss in Alzheimer-inoculated mouse lemurs. NeuN staining of the dorsal hippocampus in animals of the (a) control- (CTRL) and (b) Alzheimer-inoculated (AD) groups. CA1/2 and CA3 are highlighted. c Stereological counting revealed a reduction in the number of NeuN-positive neurons in the CA3. NeuN staining of the entorhinal cortex (EC) in control- (d) and Alzheimer-inoculated (e) groups. EC-I, EC-II, and EC-III-VI represent the different layers of the entorhinal cortex. f Stereological counting revealed a reduction in the number of NeuN-positive neurons in layers II and III-VI of the EC. NeuN staining of the cingulate/retrosplenial cortex (Cg/RS) in control- (g) and Alzheimer-inoculated (h) groups. i Stereological counting did not reveal changes of NeuN-positive neurons in the Cg/RS. Scale bars: 100 μm. *p < 0.05, Mann-Whitney test. Scatter plots display median and interquartile interval. CTRL-inoculated animals are in blue, AD1-inoculated in green and AD2-inoculated in red