Fig. 7.

Lack of glial reactivity in inoculated lemurs. a-b, Immunostaining of astrocytes (GFAP) in the hippocampus (H) and parietal cortex (P) of control (a) and Alzheimer-inoculated (b) animals. Regional differences are seen including lower GFAP-immunoreactivity in the cortices which is generally found in lemurs. No qualitative difference in astrocyte morphology was detected between control- and Alzheimer-inoculated animals. Scale bars: main frame: 1 mm; inserts: 50 μm. (c) Quantitative evaluations of astrocyte reactivity did not provide evidence of changes in GFAP-immunoreactivity or astrocyte morphology between control- and Alzheimer-inoculated animals (Mann-Whitney tests). d-f Microglia reactivity was evaluated by western blot analysis (Iba1). The unstained -UV activated- blot used for total protein amount normalization is presented in (d), while the blot probed with Iba-1 antibody showing a specific 16kD band is displayed in (e). f Quantitative evaluations of the blots did not show any difference between Iba-1 expression in control- and Alzheimer-inoculated animals (Mann-Whitney test). Scatter plots display median and interquartile interval. CTRL-inoculated animals are in blue, AD1-inoculated in green and AD2-inoculated in red. N = 6 animals per group. FC: frontal cortex; EC: entorhinal cortex; Am: amygdala; Hip: hippocampus; Cg/RS: cingulate cortex/retrosplenial cortex