Figure 6.

Decalcified histology and immunohistochemistry of the experimental group (marrow + vein perfusion), showing top to bottom: H&E at low and high magnification showing the distribution and organization of the extracellular matrix outside and within the scaffold (star = polymer clip and blue arrow = thick vascular capsule surrounding the clip); the expression of α‐smooth muscle actin (displayed in brown); type‐IV collagen distribution (in brown). Scale bars on low and high magnification represent 1 mm and 100 µm, respectively. Contrast absence of collagen IV staining inside the scaffold with the nonperfused sample in Figure 5.