FIGURE 6:

Mitochondrial translocation of p53 in myoblasts under HMS stimuli. (A) Cells were loaded under HMS for 12 and 24 h, and Mito-Tracker Red CMXRos staining was used as a marker of mitochondria before regular immunofluorescence staining. The pictures were representative results displaying p53 nuclear export and mitochondrial localization after 12 and 24 h HMS stimuli. The 0 h group was used as the control group, with the same exposure time being used for the 12 and 24 h groups. The right pictures adjacent to the left ones are magnified pictures of the white rectangular area in the left pictures. (B) WB results further confirmed p53 mitochondrial translocation in myoblasts under HMS stimuli. H3 histone, GAPDH, and Cox IV were used as markers of cytoplasmic, nuclear, and mitochondrial fractions, respectively.