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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Mol Carcinog. 2019 Jan 20;58(5):722–734. doi: 10.1002/mc.22965

Fig. 4. CWO-induced tumor regression requires cytotoxic T cells.

Fig. 4.

A. Epidermis and tumors treated with CWO or vehicle were analyzed with RNA sequencing and results were compared (left). Epidermis was treated with a single application of CWO, tumors were collected after six wks of treatment. 1577 genes were differentially enriched in skin, 215 in tumors, and 77 in both groups with CWO treatment. GO-analysis of terms enriched with CWO treatment in both papillomas and keratinocytes were analyzed (right) and compared to identify common pathways affected in both groups. Terms consistent with immune activation are in red font.

B. Tumors were induced as before, mice were split into matched cohorts, then injected with T-cell blocking or isotype control antibodies on day −1, 2 and then then 1x weekly.

C. CD8 blocking antibody reversed the effects of CWO treatment (N=7 mice/group, two-way ANOVA identified a significant interaction between time and treatment group (P<0.05), and significant effects of both time and treatment group (P<0.0001). One-way ANOVA split by treatment group, P<0.01, Tukey’s multiple comparison test

*P<0.05, **<0.01.