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. 2019 Aug 27;25:6418–6428. doi: 10.12659/MSM.917352

Figure 4.

Figure 4

KIF18A expression is upregulated in PCa cells. (A) KIF18A mRNA expression levels in PC-3, VCap, C4-2, LNCaP, and RWPE-1 cells as measured by qRT-PCR. (B) Western blotting to detect the KIF18A protein expression levels in each of the above cell lines. (C) Western blot analysis of the effects of KIF18A knockdown after transfection of PC-3 cells with the shKIF18A plasmid. (D) Western blotting was used to measure the expression levels of corresponding protein in these 2 cell lines. GAPDH was used as an endogenous reference gene.