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. 2019 Aug 28;10:2058. doi: 10.3389/fimmu.2019.02058

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Copyright © 2019 Burbano, Villar-Vesga, Vásquez, Muñoz-Vahos, Rojas and Castaño.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IL-4 does not re-polarize the proinflammatory profile of MDM from HC and patients with RA differentiated with MP and MP-IC. (A) Top, the MFI of markers associated with M1 and M2 polarizations in MDM from patients with RA (n = 6) and HC (n = 6) differentiated without (Unstim, black whisker box) or with RMP (light gray whisker box) and RMP-IC (green whisker box) along with 6 h of IL-4 treatment. Below, the MFI of markers associated with M1 and M2 polarizations in MDM from patients with SLE (n = 6) and HC (n = 6) differentiated without (Unstim, black whisker box) or with LMP (dark gray whisker box) and LMP-IC (orange whisker box) along with 6 h of IL-4 treatment. (B) Top, cytokine levels in supernatants of MDM from patients with RA (n = 6) and HC (n = 6) differentiated without (Unstim, black bar graph) or with RMP (light gray bar graph) and RMP-IC (green bar graph) along with 6 h of IL-4 treatment. Below, cytokine levels in supernatants of MDM from patients with SLE (n = 6) and HC (n = 6) differentiated without (Unstim, black bar graph) or with LMP (dark gray bar graph) and LMP-IC (orange bar graph) along with 6 h of IL-4 treatment. Comparisons among the groups were performed using ANOVA II and the Bonferroni post-hoc test.