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Effects of ESR1 KD on the expression of P450 enzymes as indicated in primary culture hepatocytes. Hepatocytes cultured with Matrigel overlay were treated with siRNA for 6 days; the mRNA levels of indicated P450 enzymes then were measured using real-time PCR with CYBR green. Compared with negative control (NC): *P < 0.05; **P < 0.01, ANOVA with Bonferroni comparison of selected pairs post test. # P<0.05 without post test.
