FIGURE 1:

The p53 apoptotic program is unresponsive in postmitotic tissue. (A) TUNEL labeling in Drosophila WT embryos and brains, with and without IR stress (scale bar = 50 µm). (B) Quantification of TUNEL positive cells (mean values represented with SD, n = 3). (C) Expression time course for indicated genes following IR stress measured by RT-ddPCR from WT head tissue (mean values represented with SD, n = 3). Note that all five genes are known p53 targets in IR-treated embryos. (D) RT-ddPCR for indicated genes 3 h post-IR in head tissue of WT and p53^−/− animals.