TABLE 2:

Viability and completion of mating for cpd2 mutant or RNAi cells.

Genotype	Viability (%)a	Mating completion (%)b
CPD2hpc	nd	97
cpd2i	nd	12
B2086d× CU428d	81	94
CU428 × cpd2cr-4	96	89
B2086 × cpd2cr-6	85	91
cpd2cr-4 × 6	2	4
cpd2cr + CPD2-HA (4–1) × (6–4)	97	85

^aViability was assayed by isolating single pairs and scoring for growth after 2 d (n = 88 pairs). nd, not determined.

^bTo check for the completion of mating, cells were fixed at 24–26 h after initiation of mating. Slides were DAPI stained and nuclear morphology was evaluated (n = 100 cells). Cells containing two SN and one GN were scored as having completed mating.

^cNoninduced RNAi strain.

^dB2086 and CU428 are the WT strains.