TABLE 2:
Human ARF GEFs.
| GEF | Localization | Function(s) | Interactors | |
|---|---|---|---|---|
| 1 | Arfgef1/BIG1 | TGN, sorting endosomes | Activation of Arf1/3, recruitment of AP1/AP3, myelination | Arl1, Arf1/3, ARF4/5 |
| 2 | Arfgef2/BIG2 | TGN, sorting endosomes | Activation of Arf1/3, recruitment of AP1/AP3 | Arl1, Arf1/3, ARF4/5 |
| 3 | Cyth1 | PM | Cell adhesion/migration, integrin regulation | GRASP/tamalin, CNKSR1-3, CASP, Arl4A, Arl4D, Arf6 |
| 4 | Cyth2/ARNO | PM, REs, Ruffles | Cell adhesion/migration, integrin regulation, actin remodeling, endosome traffic | GRASP/tamalin, CNKSR1-3, CASP, Arl4A, Arl4D, Arf6, paxillin, RLIP76, β-arrestin, pallidin |
| 5 | Cyth3/ARNO3/GRP1 | PM, (Glut4-positive) endosomes | Glut4 exocytosis, cell migration | GRASP/tamalin, CNKSR1-3, CASP, Arl4A, Arl4D, Arf6 |
| 6 | Cyth4 | |||
| 7 | GBF1 | Golgi | Membrane traffic at cis-Golgi | p115, Rab1b, COG4, γ-COP, GGA1-3, ATLG, Gmh1 |
| 8 | Iqsec1/BRAG2/GEP100 | PM | Integrin endocytosis/cell adhesion regulation of AMPA receptor traffic | Calmodulin, MAP4K4, Arf5, Arf6 |
| 9 | Iqsec2/BRAG1 | PSDs | Regulation of AMPA receptor traffic | Calmodulin, PSD95, IRSp53, Arf6 |
| 10 | Iqsec3 | PSDs | Regulation of GABAergic synapse formation | Calmodulin, gephyrin, Arf6 |
| 11 | Psd/EFA6 | PM, tight junctions, axons, PSDs, endosomes | Tight junction formation, epithelial lumen formation | α-Actinin-1, 4, Arf6 |
| 12 | Psd2/EFA6C | |||
| 13 | Psd3/EFA6D | |||
| 14 | Psd4/EFA6B | PM, epithelial tight junctions | Tight junction formation, epithelial lumen formation | α-Actinin-1, 4, Arf6 |
| 15 | Fbox8 |
NCBI gene names are listed, along with cellular localization, identified functions, and protein interactors. Abbreviations used include PM, plasma membrane; PSD, postsynaptic densities, RE, recycling endosomes; TGN, trans-Golgi network. Additional information is included in Supplemental Table II.