Movie S2.

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14-3-3 inhibits IRSp53- and Eps8- or VASP-induced FLS in live cells. This movie corresponds to cell images shown in Figure 2, and illustrates serum starved COS-7 cells expressing the following proteins: (A) GFP-Eps8, (B) GFP-Eps8 and IRSp53-mTagBFP2, (C) GFP-Eps8 and mCherry-14-3-3, (D) GFP-Eps8, IRSp53-mTagBFP2 and mCherry-14-3-3, (E) GFP-VASP, (F) GFP-VASPand IRSp53-mTagBFP2, (G) GFP-VASPand mCherry-14-3-3, (H) GFP-VASP, IRSp53-mTagBFP2 and mCherry-14-3-3. Scale bars throughout this movie represent 10 μm and 5 μm in whole-cell and inset images, respectively.