Figure 3.
OSM in combination with TNFα regulates cytokine/chemokine secretion in RAFLS and HUVEC. RAFLS and HUVEC were cultured in the presence of OSM (10 ng/ml) and increasing concentrations of TNFα (0.01, 0.1, 1 ng/ml) for 24 h. Bar graphs showing the measured secretion of IL-6, MCP-1, IL-8, GROα, and RANTES from (A) RAFLS (n = 7–10) and (B) HUVEC (n = 10–11) following the outlined treatments by ELISA. Values expressed as mean ± SEM, Wilcoxon signed rank and paired t-test were used for RAFLS and HUVEC, respectively. *p < 0.05, **p < 0.01, ***p < 0.005.