Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jun 1;30(12):1425–1436. doi: 10.1091/mbc.E18-10-0687

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Wiedmann et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.

PMC Copyright notice

FIGURE 7: — Effects of external glucose concentration on surface expression of hK_2P17.1 in HEK-293T cells. (A) Representative immunoblots of hK_2P17.1-WT channels expressed in HEK-293T cells cultured under different glucose concentrations. Input fractions (left) are provided, as well as surface fractions (right) obtained via surface biotinylation and streptavidin precipitation. Transfection state, absence or presence of biotin, and external glucose concentration are displayed. (B) Mean hK_2P17.1 protein signals in the input fractions of n = 3 independent experiments, quantified via densitometry. (C) Mean optical densities of hK_2P17.1 protein signals in the respective surface fractions. (D) Ratio of hK_2P17.1 protein signals of surface/input fractions. Data are presented as mean ± SEM. p values of two-tailed Student’s t tests vs. glucose 4.5 g/l are given as inserts.