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. 2019 Jun 1;30(12):1536–1543. doi: 10.1091/mbc.E18-12-0792

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© 2019 Pan et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 1: — In undifferentiated 3T3-L1 cells, IRAP interacts with retromer and has a long half-life. (A) Constructs used in the study. (B, C) The 3T3-L1 preadipocytes stably expressing full-length IRAP-EYFP (B) and IRAPtail-EYFP (C) were fixed, stained with the polyclonal anti-Vps35 antibody or anti-Vps26 antibody, as indicated on the panels, and analyzed under a fluorescence microscope. (D) Wild-type 3T3-L1 preadipocytes were incubated with lysotracker, fixed, stained with the monoclonal anti-IRAP antibody, and analyzed under a fluorescence microscope. (E) Wild-type 3T3-L1 preadipocytes were incubated with emetine for the indicated periods of time, and total lysates (80 µg) were analyzed by Western blotting. The graph shows quantification of the data. Error bars represent SE.