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. 2019 Jun 1;30(12):1536–1543. doi: 10.1091/mbc.E18-12-0792

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© 2019 Pan et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 3: — Knockout of Vps35 in 3T3-L1 adipocytes inhibits formation of small IRAP-containing transport vesicles. (A) Total lysates (L) prepared from wild type and Vps35-depleted (KO) 3T3-L1 adipocytes were fractionated into heavy membrane pellet (P1) and vesicular fraction (P2) that were analyzed by Western blotting (20 µg per lane). (B) A relative distribution of IRAP between fractions. The presence of each protein in total lysates (L) of wild type and Vps35-depleted 3T3-L1 adipocytes is 100%. **p < 0.01; *p < 0.05. Results of three independent experiments were analyzed by Student’s t test.