Movie S6.

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BCAS3 domain of Rudhira is essential for MT dynamic organization (related to Figure 6). Time-lapse images of SVEC KD cell line transiently overexpressing Rudhira or RudhiraΔBCAS3, incubated with SiR-Tubulin monitored for 2 minutes, imaged at 4 second intervals (6 fps). Arrow indicates persistence of radial MT at cell periphery, while asterisk indicates a MT bending before reaching cell periphery. Note the MTs criss-crossing and not aligned towards cell periphery (red box) in vector/ΔBCAS3 transfected KD cells, as compared to aligned MTs (green box) in Rudhira transfected KD cells.