FIGURE 2:

flam first exon transcripts are retained and form perinuclear foci in flam^KG/Df ovaries. (A) RT-qPCR analysis of flam transcription in flam^BG and flam^KG mutant ovaries using primer pairs for the first or third exons. Fold change of flam transcription in the ovaries with indicated genotypes relative to flam^BG/flam^BG ovaries, normalized on the Adh transcript levels, is indicated along the Y-axis (mean ± SD for two replicates). The flam third exon transcripts were barely detected in flam^BG and flam^KG mutants, but the flam first exon transcripts are retained in flam^KG mutant. (B) RNA FISH (red) with flam first exon probe coupled with immunostaining of Yb bodies with anti-Yb antibody (green) and nuclear envelope with anti-lamin Dm0 antibody (blue) in the follicle cells of the stage 2 egg chamber of flam^KG/+ (left panel), flam^KG/Df (central panel), and enlarged view of flam^KG/Df (right panel). Scale bars are 5 μm in the left and central panels and 0.7 μm in the right panel.