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. 2019 Jun 1;30(12):1406–1424. doi: 10.1091/mbc.E19-01-0008

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© 2019 Van Itallie et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 2: — Endogenous clds are variably distributed between the apical tight junction and lateral membrane in MDCK II cells. (A) Top, Maximum-intensity projections of immunofluorescence confocal colocalization of cldn2, cldn1, ZO-1, and merge (left to right panels) show that cldn1 is more laterally disposed than cldn2 or ZO-1. Bottom, Colocalization of cldn4, cldn3, ZO-1, and merge (left to right panels) reveals cldn4 and cldn3 at the lateral membrane as well as colocalized with ZO-1. Arrows in enlargement show apparent concentrations of lateral cldns likely related to lateral membrane infoldings (see Supplemental Figure S1). (B) z-Axis images as in A show ZO-1 focused at the apical junctional region and cldns colocalized with ZO-1 but also distributed along the lateral membrane.