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. 2019 Jun 1;30(12):1406–1424. doi: 10.1091/mbc.E19-01-0008

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© 2019 Van Itallie et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 5: — Cldn2 has a longer half-life than cldn4. (A) To measure half-life of SNAP-tagged proteins, MDCK cells expressing SNAP(e)cldn2 or SNAP(e)cldn4 were incubated with JF549 SNAP-tag ligand (time 0), washed and incubated with SNAP-cell block, and then collected after 3, 5, 7, and 9 h. (B) Duplicate samples of MDCK cells expressing SNAP(e)cldn 2 (top panels), SNAP(e)cldn4 (middle panels), and MDCK cells stably expressing the SNAP-tag protein alone were collected for analysis by SDS–PAGE and in-gel fluorescence. After fluorescence analysis, gels were transferred and probed for ocln as a loading control. (C) Quantification of duplicate or triplicate experiments were used to determine approximate half-lives by linear regression analysis.