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Gene expression of the components of the 5-HT synthetic pathway in third trimester FGR placentae compared with gestation-matched control placentae. Placental mRNA of TPH1 (Fig. 1A); TPH2 (Fig. 1B); SLC6A4 (Fig. 1C); and 5-HT receptors HTR5A (Fig. 1D), HTR1D (Fig. 1E) and HTR1E (Fig. 1F) relative to the geometrical averaging of the three housekeeping genes 18S rRNA, YWHAZ and TBP were determined using the 2−ΔΔCT method [32,33] and analysed using the Mann-Whitney U test.
