Skip to main content
. Author manuscript; available in PMC: 2020 Aug 17.
Published in final edited form as: ACS Synth Biol. 2019 Jul 19;8(8):1941–1951. doi: 10.1021/acssynbio.9b00229

Figure 3.

Figure 3.

Diagrams of the hapalindole-producing ‘fam’ BGC from Fischerella ambigua UTEX 1903 and the synthetic fam operons constructed on RSF1010 expression plasmids. (A) Diagram of the 42 kilobase pair gene cluster with the 32 fam genes. (B) Gene structure of plasmid pSL3228 expressing the famH genes and the lac repressor mutant W220F (‘lacI’). The famH genes were placed under control of the trc promoter surrounded by two lacO operator sequences (“trc2O”). The PmeI (MssI) restriction site was used to insert additional fam genes in subsequent constructs. (C) Operons on plasmid pSL3285. (D) Operons on plasmid pSL3251. (E) Operons on plasmid pSL3283. The plasmid backbone and the lacI cassette is omitted in all panels B – E. Terminators are indicated by black hairpins. Genes under control of the cpcB or J23119 promoter were constitutively expressed.