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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Mol Imaging Biol. 2019 Apr;21(2):200–218. doi: 10.1007/s11307-018-1239-2

Figure 4.

Figure 4.

Dual probe ex vivo specimen imaging: Representative color, fluorescence, DDSI (dual probe difference specimen imaging), H&E, and HER2 targeted IHC images of tumor and adipose tissue pairs following staining using a range of dual-stain soak concentrations and incubation times for a probe pair A (Herceptin-Cy3b, DkRb-AF647) and b probe pair B (Herceptin-AF647, DkRb-Cy3b). All images are representative of data collected for n=6 tumor and adipose tissue pairs per staining condition. All untargeted and targeted channel images are background corrected, normalized by their exposure time and calibration drop intensity. DDSI images are displayed with equivalent color scales across staining conditions. H&E and IHC images were acquired from serial sections of the same tissue face imaged in the whole specimen DDSI images. H&E: hematoxylin and eosin; IHC: immunohistochemistry. Scale bars = 5 mm. (Adapted from Barth CW, Schaefer JM, Rossi VM, Davis SC, Gibbs SL. Optimizing fresh specimen staining for rapid identification of tumor biomarkers during surgery. Theranostics 2017; 7(19):4722–4734. © Ivyspring International Publisher, 2017).