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. 2019 Aug 28;12:200. doi: 10.3389/fnmol.2019.00200

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Copyright © 2019 Sackmann, Sinha, Sackmann, Civitelli, Bergström, Ansell-Schultz and Hallbeck.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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nSMase2 was inhibited in donor cells and then treated with oα-syn (labeled with AF700) for 3 h. The donor cells were then immediately analyzed for the uptake of oα-syn or were cultured in the presence or absence of hypoxic conditions for 48 h for further analysis. Coculture experiments were carried out by placing the donor cells with oα-syn on top of fully differentiated recipient cells stably expressing β-actin-GFP. Cocultured cells were incubated in the presence or absence of hypoxic conditions for 24 h before analysis.