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. 2019 Aug 28;12:200. doi: 10.3389/fnmol.2019.00200

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Copyright © 2019 Sackmann, Sinha, Sackmann, Civitelli, Bergström, Ansell-Schultz and Hallbeck.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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nSMase2 expression is a critical component in the transmission of oα-syn between neuron-like cells. (A) EV abundance, as measured by the acetylcholinesterase (AChE) activity, of EVs isolated from donor cells treated with oα-syn (n = 4). (B,C) Flow cytometry analysis of the oα-syn (B) ratio of uptake in donor cells and (C) ratio of transfer to recipient cells after 24 h of coculture (Co) (n = 6). The provided ratios are relative to WT SH-SY5Y uptake and transfer, where the control = 1.0. (D) LDH assay demonstrating cytotoxicity after 24 h of coculture (n = 6). (E–G) Western blot analysis of (E) α-syn after 24 h of coculture and quantifications demonstrating the expression of (F) 15-kDa monomeric α-syn and (G) high MW α-syn (∼25–250 kDa) relative to that of β-actin. The data are presented as the mean ± SEM relative to the control (n = 3). ^∗p ≤ 0.05, ^∗∗p ≤ 0.01, ^∗∗∗∗p ≤ 0.0001.