Figure 2.

APα increases the nestin- and BrdU-positive cell numbers. A, BrdU incorporation was visualized by specific BrdU antibody immunofluorescence. Photomicrographic images are representative of control (top) and 500 nm APα-treated (bottom) rat E18 hippocampal cultures after 1 DIV APα exposure. Scale bars, 20 μm. B, Bar graph depicts quantitation of BrdU-positive cells from 10 randomly selected fields per slide. Total number of cells analyzed per slide was 800-1000. Three slides per condition were analyzed, for a total of 8964 cells contributing to the analysis. Data are from three independent experiments, and the results are plotted as mean ± SEM of BrdU-positive cells in percentage of vehicle group (set as 100%). ^*p < 0.05 versus vehicle.