Table 3.

Statistical summary of the effects of APα on cell-cycle gene expression using cDNA array analysis

Gene in categories	Multivariate statistic (APα vs control)	Univariate analysis (APα vs control)	Direction of change (APα vs control)
Cyclins	p < 0.05	Cyclin B, p < 0.05	Increased
		Cyclin E2, p < 0.05
CDKs	p < 0.05	Cdc2 (CDK1), p < 0.05	Increased
CDK inhibitors	p < 0.05	CDK int 16, p < 0.05	Decreased
Ubiquitin relatives	p < 0.05	Cullin 3, p < 0.05	Decreased
		Ube1X, p < 0.05
		Cullin 1, p < 0.05
PCNA	p < 0.05	PCNA, p < 0.05	Increased
Actin and GAPDH	p < 0.5	GAPDH, p < 0.5	No change

Cultures of hippocampal neurons were treated with vehicle or 500 nm APα for 24 h, followed by extraction of total RNA. Nonradioactive probes were prepared and hybridized to gene array. Data were determined by optical density and subjected to multivariate ANOVA. Ube1X, Ubiquitin-activating enzyme E1. CDK int 16, CDK inhibitor P16.