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. 2005 May 11;25(19):4755–4765. doi: 10.1523/JNEUROSCI.0553-05.2005

Figure 2.

Figure 2.

Receptor expression and electrophysiological properties of migrating neuroblasts. A-C, Immunostaining of an E17 slice cocultured for 1 DIV showing a migrating neuroblast double-labeled with antibodies against GFP (green; A and top panels in B and C) and NR1 subunit of the NMDA receptor (red; A and middle panels in B and C). Squares surrounding the soma and the tip of the leading process are shown enlarged in B and C. Green and red channels are merged in the bottom panels in B and C. D-F, Immunostaining of E17 slices cocultured for 1 DIV showing a migrating neuroblast double-labeled with antibodies against GFP (green; D and top panels in E and F) and GABAA receptor (red; D and middle panels in E and F). Squares surrounding the soma and the tip of the leading process are shown enlarged in E and F. Green and red channels are merged in the bottom panels in E and F. G, Photomicrograph of a GFP-positive migrating neuroblast recorded with a pipette filled with rhodamine. Left, GFP-positive neuroblast originated into the fluorescent slice (GFP slice) migrating onto the nonfluorescent hippocampal slice (WT slice). The arrow points the leading process, and the arrowhead points to the trailing process. Middle, The same neuroblast shown in light transmission (Light Transm.), impaled with the patch pipette. Right, The same neuroblast filled with rhodamine (Rhod.). H, Spikelet evoked by depolarizing pulses applied to a migrating neuroblast recorded with a KCl-filled pipette solution. The capacitance of the cell was 10 pF. The spikelet (indicated with an asterisk and shown at lower time scale on the right) is evoked at a membrane potential of -25 mV. I, Currents mediated by bath application of NMDA and isoguvacine and recorded at 30 mV in the same cell (Cm, 6 pF) with a CsGlu-filled pipette solution. Both receptor-mediated currents are blocked by their respective antagonists. J, Migrating neuroblast (Cm, 3 pF) recorded at 30 mV with a CsGlu-filled pipette solution. Bath application of GABAA receptor antagonists generates an inward current associated with a decrease of the basal noise. Depolarizing pulses (5 mV, 10 ms, applied every 10 s) show that these effects are not associated with changes in the serial resistance. The bottom traces show representative responses to the current pulse before (a) and at the plateau (b) of the tonic current. Scale bars: A, D, 20 μm; B, E, 5 μm; C, F, 2 μm; G, 10 μm.