Figure 6.

GABA_A and NMDA receptor antagonists impaired the migration of BrdU-labeled cells in hippocampal organotypic slice culture from munc18-1 mutant mice. A, Immunostaining for BrdU on hippocampal organotypic slices from munc18-1 mutant mice cultured for 1 DIV without any treatment [control condition (munc); left], in the presence of 10 μm MK801 (middle), and in the presence of 50 μm bicuculline (right). In the absence of any treatment in munc18-1 hippocampal slices, the BrdU+ cells after 1 DIV are distributed partly into the migration area [i.e., neuroepithelium (ne)/stratum oriens (s. or.)] and partly into the stratum pyramidale (s.pyr.). After 1 DIV in the presence of MK801 and to a greater extent in the presence of bicuculline, BrdU+ cells that have failed to migrate are distributed into the migration area. Scale bar, 20 μm. B, Histogram showing the migration indices obtained after treatment for 1 DIV with 10 μm MK801 or 50 μm bicuculline compared with the untreated control (munc) condition in hippocampal organotypic slice cultures from munc18-1 mutant mice. The migration indices are expressed (± SEM) as the ratio between the percentage of cells that reached the stratum pyramidale after 1 DIV and the percentage of cells that were still in the migration area (i.e., from the neuroepithelium to the stratum oriens), with the average of the control values being set to 10. ^#p < 0.01. The number of experiments is given in parentheses. CTRL, Control; BICU, bicuculline.