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. 2005 May 11;25(19):4694–4705. doi: 10.1523/JNEUROSCI.0311-05.2005

Figure 4.

Figure 4.

Acute transplantation of hESC-derived OPCs resulted in a significant increase in the density of oligodendrocyte remyelination compared with controls. a, Electron micrograph of the transplant environment at 7 d after injury, illustrating demyelinated axons (*) in an extracellular environment free of astrogliosis. b, Toluidine blue-stained transverse section and electron micrograph (c), illustrating robust oligodendrocyte remyelination (R; with characteristically thin myelin sheaths) among few normally myelinated axons (N). d, e, Anti-GFP and anti-neurofilament double immunostains illustrating highly branched GFP-positive OPCs (O) extending processes that ensheath nearby neurofilament-positive axons (arrows), confirming that remyelination was performed by eGFP-labeled transplanted cells. f, Quantification of normally myelinated, demyelinated, and oligodendrocyte or Schwann cell-remyelinated axons in hESC-derived OPC-transplanted, hFb-transplanted, and DMEM-injected animals. Error bars illustrate SD. The myelin sheath thickness for each class of axons is indicated in brackets. Magnification: a, 6000×; b, 400×; c, 3000×; d, 600×; e, 1000×.