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. 2005 Jun 22;25(25):5975–5983. doi: 10.1523/JNEUROSCI.0804-05.2005

Figure 1.

Figure 1.

ELKS is an active zone protein in C. elegans. A, ELKS and RAB3 immunostaining in C. elegans. Whole mounts of wild-type C. elegans fixed and prepared for immunohistochemical detection of ELKS and RAB-3 proteins in the nerve ring (NRing), ventral nerve cord (VCord), and dorsal nerve cord (DCord). ELKS immunostaining is found in synapse-rich regions of the C. elegans nervous system and along the pharyngeal basal lamina. Autofluorescent background of bacteria within the lumen can also be seen. B, ELKS and RIM colocalize in C. elegans neurons. Whole mounts of wild-type C. elegans fixed and prepared for immunohistochemical detection of ELKS (red) and RIM (green) proteins in the dorsal nerve cord. C, ELKS C-terminal tail binds the PDZ domain of RIM. Western blots using ELKS antibodies revealed that recombinant ELKS C-terminal tail (last 126 amino acids) tagged to His6 bound to recombinant GST-PDZRIM (containing amino acids 627-974 of RIM) but not to GST alone. NB, Nonbound fractions; B, bound fractions. Data are representative of three experiments. Scale bars, 20 μm.