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. 2005 Jun 22;25(25):5956–5966. doi: 10.1523/JNEUROSCI.0880-05.2005

Figure 8.


Figure 8.

Regional adenosine A1 receptor levels in the hippocampus of rats of different ages. A, B, Low-magnification photomicrographs of immunostaining for the A1 receptor in sections through the hippocampus of 2-month-old (A) and 9-month-old (B) rats shows comparably dense immunostaining in the CA1 str. oriens (SO) and str. radiatum (SR). The arrow indicates the approximate CA1/CA2 boundary. Scale bar: (in A) A, B, 500 μm. C, Representative Western blot shows levels of PSD-95 (top) and A1 receptor (A1R; bottom) immunoreactivities in homogenates from the CA1 str. radiatum (SR) and str. oriens (SO) from young adult (1-2 months of age) and middle-aged (8-10 months of age) rats (10 μg of protein per lane; immunoreactive bands were 34 and 89 kDa, respectively). D, E, Graphs show levels of A1 receptor (D) and PSD-95 (E) immunoreactivities assessed by densitometric analysis of Western blot ECL films (n = 6 rats/age).