Figure 5.
PINTA functions downstream of vitamin A production. A, Normal Rh1 levels are restored in ninaDP246 flies (Stephenson et al., 1983) but not in pinta flies by feeding with vitamin A (VA). Both ninaDP246 and pinta flies were fed either normal food or instant food with 10 μg/ml all-trans-retinal. A Western blot containing fly head extracts (2 d after eclosion) was probed with anti-Rh1 or anti-tubulin antibodies. B-F, PDAs performed using the same paradigm as in Figure 1 A. Flies were fed normal food unless indicated otherwise. B, Wild type; C, vitamin A-fed ninaD; D, ninaD; E, vitamin A-fed pinta; F, pinta. O, Orange light; B, blue light.